“ Simple randomization was used in each of the four trial groups to allocate patients to the trial arms with a block size of four lasix loop diuretic In addition, a recent study has shown that leptin was produced by breast cancer cells Tessitore et al, 2000, and it is possible that levels of circulating leptin in subjects for whom samples were collected subsequent to breast cancer diagnosis and or receiving adjuvant treatments may be influenced by the disease process and or treatment protocol ”